In-vitro-Biology Services
- Piramal’s in vitro Biology is designed to provide best in class support to our clients to enable their preclinical drug discovery, including assay development, hit identification, hit-to-lead and lead optimization, including HTS, multiplex assay development, primary/secondary functional assays, HCS, and selectivity.
- Our assay team can develop, and screen compounds, with miniaturization of assays achieved by using 96 and 384-well plates. Our assays are validated rigorously using tool compounds with QC established (signal: noise ratio; Z’-prime;uniformity; reproducibility).
Capabilities
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In vitro Biology offers high quality screening through-
- Target based screening
- Enzymes (kinases, proteases, phosphatases, dehydrogenases etc.)
- GPCR
- Ion Channels
- Nuclear Receptor
- Protein-protein interaction
- Phenotype based screening (based on the therapeutic Indication)
- Target based screening
- Experienced team well versed in biochemistry, cell biology, HTS, HCS and protein engineering
- Dedicated instrumentation to provide evaluation across multiple formats such as fluorescence (HTRF,FP FI), luminescence, alphascreen and MS
- Compound Storage and Management with cryo-storage and distribution systems
- All of our service offerings are available under one roof, giving you a seamless experience
Some of the key instruments are listed below:
- Liquid handling systems/Pipetting stations
- Echo 650(Acoustic Dispensing)
- Integra
- Biomek i7 (Beckman Coulter) integrated with PHERAstar
- 96-well microplate washer
- Multimode plate readers
- Polarstar (BMG Labtech)
- CLARIOstar(BMG Labtech)
- PHERAstar FSX (BMG Labtech)
- FLIPR Penta
- High Content Screening Instruments
- ImageXpress Confocal Microscope HT.ai (Molecular Devices)
- Incucyte SX5
- Bioanalytical
- HPLCs (Agilent, Shimadzu)
- LC-MS/MS